Characterization of RNA in Extracellular Vesicles

Extracellular vesicles (EVs) are important players in the communication between different kinds of cells by delivering their content, consisting types RNA, proteins, bioactive lipids, or signaling nucleotides, into target cells. Several EVs distinguished: (1) exosomes with sizes from 30 to 150 nm originate endosomal pathway and form intracellular multivesicular bodies (MVBs), which fuse plasma membrane before secretion. (2) ranging 100 1000 diameter formed during cell surface budding. (3) Apoptotic diameters 500 2000 released blebbing apoptotic It is well established that various RNA molecules such as coding RNAs noncoding (long RNAs, microRNAs, circular rRNAs) present amounts depending on type origin EV. Here we will give an overview methods isolate quantify characterize species.